Not Competing : 1
| Heat Number - 1 | |||||
| Lane | Comp.No. | Name | AaD | Club/Country | Time |
| 1 | 2 | Aimee SMITH | 12 | Bolton Metro | 39.40 |
| 2 | 4 | Katie GREENWOOD | 15 | SwimTrafford | 36.54 |
| 3 | 6 | Laura HYLAND | 15 | Southport | 35.40 |
| 4 | 8 | Danielle LOWE | 14 | LiverpoolPen | 33.70 |
| 5 | 7 | Keri-Anne PAYNE | 21 | Stockport Mo | 35.00 |
| 6 | 5 | Jessica CHADWICK | 14 | Preston | 35.52 |
| 7 | 3 | Lucy SPENCER | 16 | Co Sheffield | 37.30 |