Not Competing : 7,13,25,44,48
| | | Heat Number - 1 | | | |
| Lane | Comp.No. | Name | AaD | Club/Country | Time |
| 3 | 2 | Lucy ROWLAND | 12 | Burnley BOBC | 5:16.00 |
| 4 | 4 | Laura MULLANY | 12 | Stockport Mo | 5:09.00 |
| 5 | 3 | Emma BURNS | 11 | Bolton Metro | 5:14.52 |
| 6 | 1 | Ellie BAGSHAW | 11 | Stockport Mo | 5:22.78 |
| | | Heat Number - 2 | | | |
| Lane | Comp.No. | Name | AaD | Club/Country | Time |
| 1 | 6 | Florence THWAITES-MILE | 11 | Stockport Mo | 5:03.92 |
| 2 | 9 | Lauren MILLS | 12 | Stockport Mo | 5:00.84 |
| 3 | 11 | Olivia ASKEW | 11 | Oldham Aqua | 4:58.19 |
| 4 | 14 | Laura HIBBERT | 12 | Stockport Mo | 4:56.90 |
| 5 | 12 | Gabrielle BUTTERWORTH | 13 | SwimTrafford | 4:57.70 |
| 6 | 10 | Sammie-Jo NIGHTINGALE | 12 | Stockport Mo | 4:59.66 |
| 7 | 8 | Emily BARCLAY | 12 | Bolton Metro | 5:02.59 |
| 8 | 5 | Nicola FARRELL | 12 | Oldham Aqua | 5:04.89 |
| | | Heat Number - 3 | | | |
| Lane | Comp.No. | Name | AaD | Club/Country | Time |
| 1 | 16 | Lillie TEW-WEAVER | 12 | Stockport Mo | 4:52.88 |
| 2 | 18 | Georgia EGAN | 14 | Bolton Metro | 4:51.05 |
| 3 | 20 | Charlotte ASHWORTH | 14 | Oldham Aqua | 4:51.00 |
| 4 | 22 | Holly MADDEN | 14 | Stockport Mo | 4:48.72 |
| 5 | 21 | Vicky CUNNINGHAM | 15 | Winsford | 4:49.12 |
| 6 | 19 | Hannah CLEGG | 14 | Oldham Aqua | 4:51.00 |
| 7 | 17 | Heather LAWSON | 14 | SwimTrafford | 4:51.86 |
| 8 | 15 | Jessica GALLIMORE | 14 | Winsford | 4:55.97 |
| | | Heat Number - 4 | | | |
| Lane | Comp.No. | Name | AaD | Club/Country | Time |
| 1 | 24 | Shannon CLARK | 14 | Satellite | 4:48.56 |
| 2 | 27 | Rachel HEESOM | 14 | Warrington W | 4:48.17 |
| 3 | 29 | Danielle LOWE | 14 | LiverpoolPen | 4:46.00 |
| 4 | 31 | Sarah HOYE | 17 | SwimTrafford | 4:43.71 |
| 5 | 30 | Alexandra RIACH | 13 | Stockport Mo | 4:44.27 |
| 6 | 28 | Kiera WELLS | 14 | SwimTrafford | 4:46.80 |
| 7 | 26 | Ambah SIMPSON | 14 | Stockport Mo | 4:48.34 |
| 8 | 23 | Laura BROWN | 15 | Stockport Mo | 4:48.62 |
| | | Heat Number - 5 | | | |
| Lane | Comp.No. | Name | AaD | Club/Country | Time |
| 1 | 33 | Charlotte CAIN | 14 | Wigan BEST | 4:43.00 |
| 2 | 35 | Emily KNOWLES-JONES | 13 | Stockport Mo | 4:42.57 |
| 3 | 37 | Karlie STOCKDALE | 14 | Barrow | 4:39.12 |
| 4 | 39 | Katie BENNETT | 14 | Satellite | 4:37.86 |
| 5 | 38 | Tanya LANCASTER | 17 | Wigan BEST | 4:38.65 |
| 6 | 36 | Sophie PYATT | 14 | Stockport Mo | 4:41.67 |
| 7 | 34 | Kate YEOMAN | 14 | Co Sheffield | 4:42.59 |
| 8 | 32 | Ellie NEWBY | 15 | Halton | 4:43.30 |
| | | Heat Number - 6 | | | |
| Lane | Comp.No. | Name | AaD | Club/Country | Time |
| 1 | 41 | Lucy GRAINGE | 15 | SwimTrafford | 4:33.84 |
| 2 | 43 | Abbie HENDERSON | 17 | Stockport Mo | 4:32.92 |
| 3 | 46 | Catherine MARCROFT | 17 | Stockport Mo | 4:20.32 |
| 4 | 49 | Eleanor FAULKNER | 16 | Co Sheffield | 4:11.92 |
| 5 | 47 | Helen MCROBERTS | 18 | Warrender Ba | 4:19.10 |
| 6 | 45 | Alice MCALL | 18 | Wigan BEST | 4:24.01 |
| 7 | 42 | Ashleigh DALE | 15 | Barrow | 4:33.21 |
| 8 | 40 | Emma RATE | 15 | Stockport Mo | 4:35.39 |