Not Competing : 5,17
| | | Heat Number - 1 | | | |
| Lane | Comp.No. | Name | YoB | Club/Country | Time |
| 3 | 1 | Emma BURNS | 98 | Bolton Metro | 36.42 |
| 4 | 3 | Olivia ASKEW | 97 | Oldham Aqua | 35.07 |
| 5 | 2 | Katie NIGHTINGALE | 97 | Co Salford | 35.26 |
| | | Heat Number - 2 | | | |
| Lane | Comp.No. | Name | YoB | Club/Country | Time |
| 1 | 6 | Faye HENESY | 95 | Nova Cent'n | 32.88 |
| 2 | 8 | Paige BOWDEN | 95 | Crosby | 32.50 |
| 3 | 10 | Emma RUSHWORTH | 95 | Swim Flint | 31.78 |
| 4 | 12 | Clare RIDLER | 93 | Satellite | 31.60 |
| 5 | 11 | Kimberley ROBINSON | 95 | Stockport Mo | 31.61 |
| 6 | 9 | Marsha BANKS | 93 | Burnley BOBC | 32.02 |
| 7 | 7 | Francesca CHIAPPE | 96 | Oldham Aqua | 32.50 |
| 8 | 4 | Kerry HOLDING | 96 | Burnley BOBC | 33.85 |
| | | Heat Number - 3 | | | |
| Lane | Comp.No. | Name | YoB | Club/Country | Time |
| 1 | 14 | Tegan TURNER-CLARKE | 95 | Co Sheffield | 31.42 |
| 2 | 16 | Rebecca TURNER | 92 | Co Sheffield | 30.86 |
| 3 | 19 | Fiona DONNELLY | 95 | Nova Cent'n | 29.15 |
| 4 | 21 | Lucy WORRALL | 92 | Stockport Mo | 28.40 |
| 5 | 20 | Danielle LOWE | 95 | LiverpoolPen | 28.49 |
| 6 | 18 | Charlotte WOOLISCROFT | 90 | Shiverers | 29.43 |
| 7 | 15 | Jess KENDALL | 94 | Co Sheffield | 31.20 |
| 8 | 13 | Tanya LANCASTER | 92 | Wigan BEST | 31.50 |